Harmonization of antimicrobial susceptibility testing by broth microdilution for Rhodococcus equi of animal origin.
نویسندگان
چکیده
genes. The PCR-based replicon typing method showed that this blaNDM-1-positive plasmid belonged to the IncHI incompatibility group. Genetic structures surrounding the blaNDM-1 gene were investigated using a PCR mapping approach and sequencing. The blaNDM-1 gene was immediately bracketed by a truncated insertion sequence ISAba125 upstream and the bleomycin resistance gene bleMBL downstream. This genetic structure was similar to those observed surrounding the blaNDM-1 gene in Enterobacteriaceae. 7,10
منابع مشابه
MICs of 32 antimicrobial agents for Rhodococcus equi isolates of animal origin.
OBJECTIVES The aim of this study was to determine the MICs of 32 antimicrobial agents for 200 isolates of Rhodococcus equi of animal origin by applying a recently described broth microdilution protocol, and to investigate isolates with distinctly elevated rifampicin MICs for the genetic basis of rifampicin resistance. METHODS The study included 200 R. equi isolates, including 160 isolates fro...
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MICs of daptomycin, linezolid, and vancomycin against 212 isolates, including Listeria monocytogenes and Pediococcus, Leuconostoc, Rhodococcus, and Nocardia spp., were determined by the broth microdilution method; daptomycin MICs were also determined by the Etest. Except with those for Leuconostoc spp., daptomycin Etest MICs showed >90% agreement with MICs obtained by the broth microdilution me...
متن کاملComparison of Etest, disk diffusion, and broth macrodilution for in vitro susceptibility testing of Rhodococcus equi.
MICs of erythromycin, clarithromycin, azithromycin, rifampin, gentamicin, and doxycycline against 101 isolates of Rhodococcus equi were determined by broth macrodilution, disk diffusion, and Etest. Categorical agreement ranged between 85.1 and 100%. Overall, the agreement between Etest and disk diffusion was better than the agreement between broth macrodilution and the agar-based methods.
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ورودعنوان ژورنال:
- The Journal of antimicrobial chemotherapy
دوره 68 9 شماره
صفحات -
تاریخ انتشار 2013